cell membrane green fluorescence staining kit  (Beyotime)

Journal: European Journal of Medical Research

Article Title: M2 macrophage-derived exosomes reverse TGF-β1-induced epithelial mesenchymal transformation in BEAS-2B cells via the TGF-βRI/Smad2/3 signaling pathway

doi: 10.1186/s40001-025-02516-4

Figure Lengend Snippet: Characterization of M2Φ-exos. A Morphology of M2Φ-exos under TEM (scale bar, 100 nm). B Immunoblot analysis of CD81 and TSG101 expression levels in M2Φ-exos and the cell lysate. C Distribution range and concentration of M2Φ-exos detected by NTA. D Representative image of M2Φ-exos taken by fluorescence microscopy after 3 h. BEAS-2B cells were incubated with M2Φ-exos labeled with the green-colored PKH67 fluorescent probe. Nuclei were labeled with blue-colored 4′,6-diamidino-2-phenylindole (scale bar, 200 μm)

Article Snippet: The PKH67 Green Fluorescent Cell Linker Kit and 4′,6-diamidino-2-phenylindole (DAPI) were acquired from Sigma‒Aldrich (Missouri, USA), while phosphotungstic acid hydrate was purchased from Beijing Solarbio Science & Technology Co., Ltd. (Beijing, China).

Techniques: Western Blot, Expressing, Concentration Assay, Fluorescence, Microscopy, Incubation, Labeling

Journal: Frontiers in Bioscience-Landmark

Article Title: Atomized Neutrophil Membrane-coated MOF Nanoparticles for Direct Delivery of Dexamethasone for Severe Pneumonia

doi: 10.31083/fbl26721

Figure Lengend Snippet: Fig. 5. In vitro and in vivo targeting. (A) Confocal fluorescence imaging of DiO-labeled NMPs after incubation with activated macrophages (blue, nuclei; green, UiO66-Dex@NMP). Scale bar = 40 µm. (B) Fluorescence quantification of UiO66-Dex@NMP per

Article Snippet: The BCA protein assay kit, Cell Membrane Green Fluorescence Staining Kit (DiO), EISA kits for mouse tumor necrosis factor-α (TNF-α) (P5318, Beyotime, Shanghai, China) and inflammatory factors interleukin 6 (IL-6) (PI335, Beyotime, Shanghai, China) were purchased fromShanghai BeyotimeBiotechnology (Shanghai, China).

Techniques: In Vitro, In Vivo, Fluorescence, Imaging, Labeling, Incubation

Journal: Advanced Science

Article Title: Human Amniotic Epithelial Stem Cells Promote Colonic Recovery in Experimental Colitis via Exosomal MiR‐23a–TNFR1–NF‐κB Signaling

doi: 10.1002/advs.202401429

Figure Lengend Snippet: Characterization and effects of hAESC‐derived exosomes. a) NIR imaging and quantification of Dil dye‐labeled hAESCs following anal injections. NIR signals were captured from the five main organs (lungs, liver, spleen, kidneys, and large bowel) and corresponding MLNs daily post‐injection. b) Morphology of exosomes visualized via transmission electron microscopy. Scale bar = 100 nm. c) Particle size distribution of exosomes determined via nanoparticle tracking analysis (NTA). d) Western blotting analysis of exosome‐specific markers. e) Uptake of exosomes by FHC cells. FHC cells were incubated with PKH67‐labeled hAESC‐exos and imaged at 24 h via confocal microscopy. Scale bars = 10 µm. f) Evaluation of the wound healing ability of hAESC‐exo‐treated and untreated FHC cells under inflammatory stimuli at 24 h; quantification summary is shown on the right side. Scale bars = 100 µm. g) Assessment of adhesion between THP‐1 and FHC cells under inflammatory stimuli with or without pre‐incubation with hAESC‐exos; quantification summary is shown on the right side. Scale bars = 100 µm. h) H&E staining of colon tissue sections (left) and assessment of histological scores (right). Scale bar = 100 µm. Data are represented as the mean ± SEM. Unpaired t ‐test or one‐way ANOVA followed by Tukey's post‐hoc test were used for analysis. n = 5. **** p < 0.0001, ** p < 0.01, and * p < 0.05; ns, not significant ( p > 0.05).

Article Snippet: Furthermore, uptake of exosomes was visualized using the PKH67 Green Fluorescent membrane linker dye (Solarbio, Beijing, China), following the manufacturer's instructions.

Techniques: Derivative Assay, Imaging, Labeling, Injection, Transmission Assay, Electron Microscopy, Western Blot, Incubation, Confocal Microscopy, Staining